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Antibodies sure to the blocking peptide not bind to the epitope to the goal protein. By evaluating the staining through the blocked antibody versus the antibody by yourself, one can see which staining is precise.
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This guideline outlines more probable optimization of cellular and immunoassay parameters to guarantee the best possible benefits are...
This guide outlines more probable optimization of cellular and immunoassay parameters to be sure the very best results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind especially towards the goal antibody and block antibody binding. These peptide generally contains the epitope recognized by the antibody.
Target Info Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary which is degraded as cells progress through S period.
Overexpression of this gene has long been noticed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was located to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the here ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential function in advertising and marketing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been described. Two added splice variants were documented but in-depth nucleotide sequence info will not be yet obtainable.
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